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phusion high fidelity master mix  (New England Biolabs)


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    Structured Review

    New England Biolabs phusion high fidelity master mix
    Phusion High Fidelity Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6470 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phusion+high+fidelity+pcr+master+mix/Phusion+High-Fidelity+PCR+Master+Mix+with+HF+Buffer/pmc13691489-72-22-26
    Average 99 stars, based on 6470 article reviews
    phusion high fidelity master mix - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Flavor development in vacuum dry-salted cowpeas: changes in volatile compounds and non-volatile metabolites during fermentation
    Article Snippet: .. PCR amplification was conducted with Phusion High-Fidelity PCR Master Mix (New England Biolabs). ..

    Article Title: A cold-adapted Cas9 nuclease from the deep ocean
    Article Snippet: Following incubation for 1 h at room temperature, 10μl of the ligation reaction was amplified using Q5 DNA polymerase (New England Biolabs; 15 cycles) with primers specific to the PAM-proximal region of the library and the ligated adapter (Supplementary Data 3). .. PCR products were purified, and Illumina sequencing adapters and sample indices were incorporated through two successive rounds of PCR using Phusion High-Fidelity PCR Master Mix in HF buffer (New England Biolabs; 10 cycles per round). .. Libraries were sequenced on an Illumina MiSeq instrument with a 25% (v/v) PhiX Control v3 spike-in.

    Article Title: How Bacterial Growth-Phase Reshapes Synthetic Gene Circuit Function
    Article Snippet: DNA oligos and genes were ordered using the Oligo Rapid Service from Azenta Life Sciences. .. DNA fragments to be assembled were amplified by PCR using Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB, M0532S). ..

    Article Title: Yupingfeng powder attenuates influenza A virus induced lung injury via gut microbiota-dependent modulation of branched-chain amino acid metabolism and macrophage polarization
    Article Snippet: .. PCR amplification was performed using barcode-specific primers, Phusion High-Fidelity PCR Master Mix with GC Buffer, and efficient high-fidelity enzymes (New England Biolabs, Beijing, China). .. SMRT Bell libraries were constructed, and sequencing was performed on the PacBio platform by Novogene Co., Ltd. (Beijing, China) following standard protocols.

    Article Title: Analysis of rumen fermentation, bacterial diversity and blood biochemical parameters in sheep fed with cotton straw and different roughage combinations
    Article Snippet: .. The polymerase chain reaction (PCR) amplification used specific barcoded primers: forward primer 338F (5′-ACTCCTACGGGAGGCAGCAG-3′) and reverse primer 806R (5′-GGACTACHVGGGTWTCTAAT-3′) with the Phusion High-Fidelity PCR master mix with GC buffer (New England Biolabs). ..

    Article Title: 16S rRNA Datasets of Gut Microbiota in Stent-Based Diverting Technique and Ileostomy from A Prospective, Open-Label, Multicenter Randomized Controlled Trial
    Article Snippet: .. All PCR reactions were carried out with 15 μL of Phusion High-Fidelity PCR Master Mix (New England Biolabs); 0.2 μM of forward and reverse primers, and about 10 ng template DNA. ..

    Article Title: Targeted Gastrointestinal Delivery of mRNA Lipid Nanoparticles Enables Systemic Vaccination and Glucagon-like Peptide-1 Metabolic Therapy
    Article Snippet: .. Linear DNA templates for IVT were amplified by PCR using Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs), forward primer, and a T120-containing reverse primer. .. The amplified PCR products were purified with the DNA Clean & Concentrator Kit (Zymo Research).

    Article Title: Pediococcus acidilactici PA53 Supplementation Improves Stool Form and Modulates Gut Microbiota and Inflammatory Responses in Adults With Functional Constipation: A Randomized, Double‐Blind, Placebo‐Controlled Trial
    Article Snippet: .. PCR amplification (30 μL reactions) contained 15 μL of Phusion High‐Fidelity PCR Master Mix (New England Biolabs, USA), 0.2 μM of each primer and 10 ng of template DNA. .. Thermal cycles: initial denaturation at 98°C for 1 min; 30 cycles of denaturation at 98°C for 10 s, annealing at 50°C for 30 s, extension at 72°C for 30 s; final extension at 72°C for 5 min. Amplicons were run on 2% agarose gels, purified using the Qiagen Gel Extraction Kit and quantified using a Qubit 2.0 fluorometer.

    Amplification:

    Article Title: Flavor development in vacuum dry-salted cowpeas: changes in volatile compounds and non-volatile metabolites during fermentation
    Article Snippet: .. PCR amplification was conducted with Phusion High-Fidelity PCR Master Mix (New England Biolabs). ..

    Article Title: How Bacterial Growth-Phase Reshapes Synthetic Gene Circuit Function
    Article Snippet: DNA oligos and genes were ordered using the Oligo Rapid Service from Azenta Life Sciences. .. DNA fragments to be assembled were amplified by PCR using Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB, M0532S). ..

    Article Title: Yupingfeng powder attenuates influenza A virus induced lung injury via gut microbiota-dependent modulation of branched-chain amino acid metabolism and macrophage polarization
    Article Snippet: .. PCR amplification was performed using barcode-specific primers, Phusion High-Fidelity PCR Master Mix with GC Buffer, and efficient high-fidelity enzymes (New England Biolabs, Beijing, China). .. SMRT Bell libraries were constructed, and sequencing was performed on the PacBio platform by Novogene Co., Ltd. (Beijing, China) following standard protocols.

    Article Title: Analysis of rumen fermentation, bacterial diversity and blood biochemical parameters in sheep fed with cotton straw and different roughage combinations
    Article Snippet: .. The polymerase chain reaction (PCR) amplification used specific barcoded primers: forward primer 338F (5′-ACTCCTACGGGAGGCAGCAG-3′) and reverse primer 806R (5′-GGACTACHVGGGTWTCTAAT-3′) with the Phusion High-Fidelity PCR master mix with GC buffer (New England Biolabs). ..

    Article Title: Targeted Gastrointestinal Delivery of mRNA Lipid Nanoparticles Enables Systemic Vaccination and Glucagon-like Peptide-1 Metabolic Therapy
    Article Snippet: .. Linear DNA templates for IVT were amplified by PCR using Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs), forward primer, and a T120-containing reverse primer. .. The amplified PCR products were purified with the DNA Clean & Concentrator Kit (Zymo Research).

    Article Title: Pediococcus acidilactici PA53 Supplementation Improves Stool Form and Modulates Gut Microbiota and Inflammatory Responses in Adults With Functional Constipation: A Randomized, Double‐Blind, Placebo‐Controlled Trial
    Article Snippet: .. PCR amplification (30 μL reactions) contained 15 μL of Phusion High‐Fidelity PCR Master Mix (New England Biolabs, USA), 0.2 μM of each primer and 10 ng of template DNA. .. Thermal cycles: initial denaturation at 98°C for 1 min; 30 cycles of denaturation at 98°C for 10 s, annealing at 50°C for 30 s, extension at 72°C for 30 s; final extension at 72°C for 5 min. Amplicons were run on 2% agarose gels, purified using the Qiagen Gel Extraction Kit and quantified using a Qubit 2.0 fluorometer.

    Purification:

    Article Title: A cold-adapted Cas9 nuclease from the deep ocean
    Article Snippet: Following incubation for 1 h at room temperature, 10μl of the ligation reaction was amplified using Q5 DNA polymerase (New England Biolabs; 15 cycles) with primers specific to the PAM-proximal region of the library and the ligated adapter (Supplementary Data 3). .. PCR products were purified, and Illumina sequencing adapters and sample indices were incorporated through two successive rounds of PCR using Phusion High-Fidelity PCR Master Mix in HF buffer (New England Biolabs; 10 cycles per round). .. Libraries were sequenced on an Illumina MiSeq instrument with a 25% (v/v) PhiX Control v3 spike-in.

    Gas Chromatography:

    Article Title: How Bacterial Growth-Phase Reshapes Synthetic Gene Circuit Function
    Article Snippet: DNA oligos and genes were ordered using the Oligo Rapid Service from Azenta Life Sciences. .. DNA fragments to be assembled were amplified by PCR using Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB, M0532S). ..

    Article Title: Yupingfeng powder attenuates influenza A virus induced lung injury via gut microbiota-dependent modulation of branched-chain amino acid metabolism and macrophage polarization
    Article Snippet: .. PCR amplification was performed using barcode-specific primers, Phusion High-Fidelity PCR Master Mix with GC Buffer, and efficient high-fidelity enzymes (New England Biolabs, Beijing, China). .. SMRT Bell libraries were constructed, and sequencing was performed on the PacBio platform by Novogene Co., Ltd. (Beijing, China) following standard protocols.

    Article Title: Analysis of rumen fermentation, bacterial diversity and blood biochemical parameters in sheep fed with cotton straw and different roughage combinations
    Article Snippet: .. The polymerase chain reaction (PCR) amplification used specific barcoded primers: forward primer 338F (5′-ACTCCTACGGGAGGCAGCAG-3′) and reverse primer 806R (5′-GGACTACHVGGGTWTCTAAT-3′) with the Phusion High-Fidelity PCR master mix with GC buffer (New England Biolabs). ..



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